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RESEARH
FLOW CYTOMETRY CORE FACILITY
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About
the Laboratory...
The Core facility laboratory is a nonprofit joint venture between
the School of Medicine and University Hospital. It is directed by
Dr. Marc Golightly and supervised by Ms. Leombruno. The Laboratory
is part of the Clinical Immunology Laboratories and is housed in University
Hospital. The laboratory is equipped with two Becton Dickinson FACSCAN
analyzers (Argon laser), two FACS Caliburs (argon & red diode
lasers) and a three laser Cell Sorter ( A brand new state-of-the-art
FACS ARIA with full supportive equipment). Six full time technologists
staff it |
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Core
Facility Services:
 Consultative
Services
- Experimental setup/review, analysis interpretation, manuscript
review
Rate
and Fee Structure - Rates for services /instruments/ analysis
& Billing information
Scheduling
Facility Time - Policies, Internet scheduling, phone scheduling
Links
to other Flow Cytometry Core Facilities
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The Flow
Cytometry Crew
(L to R, Corinne, Nancy, Todd, Chris, Kerri
not pictured: Becky, James, Sandy, & Nikki)
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THE NEW FACS ARIA IS HERE!
FASTER, MORE SENSITIVE, MICROTITER PLATE SORTING, BIOHAZARD CONTAINMENT,
THREE LASERS, 4 WAY 8 PARAMETER SORTS, 8 COLOR ANALYSIS.
Here are common dyes
and wavelengths used for FACS ARIA:
488
laser
515-545nm FITC
564-606nm or 563-589nm PE
600-620nm PE-Texas Red®
675-715nm or 665-685nm PE-Cy5 or PerCP or PI
750-810nm PE-Cy7
633 laser
650-670nm APC
750-810nm APC-Cy7
405 laser
515-545nm Alexa Fluor® 430 (can not be used if FITC is used)
430-470nm Pacific Blue™ or DAPI or Hoechst
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| Consultative
Services: |
- Free consultation
to discuss the application of Flow Cytometry to the principal
investigator's project or proposed use. All users are encouraged
(required) to do this . This consultation often provides the PI
alternatives, insures proper controls will be run, and often eliminates
repeat studies due to technical inexperience with Flow Cytometry.
- Free review
of data presentation for publication. It has been published that
a large percentage of published Flow Cytometry data is presented
wrong or erroneous conclusion have been made. This service seeks
to eliminate errors in data presentation/conclusions thus increasing
the chances for manuscript acceptance.
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| Rate
and Fee Structure: |
- Rates are
charged in 1/4 hour increments according to the rate structure
below
- A setup
charge occurs with each use (see setup times below), therefore
it is advantageous to batch your use if at all possible.
- For those
fimiliar with operation of FACScan/Calibur, we offer investigator
use rates. If not, we will train you at normal rates (it usually
takes 2-8 hours, depending on the individual).
- Bills are
sent to the principle investigator quarterly and are billed to
the PI's grant number. Please advise us of changes in funding
status or desire to bill another account.
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INSTRUMENT/SERVICE
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SUNY
STONY BROOK
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NON
- SUNY STONY BROOK
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Computer
Data Analysis
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$32 per hour |
$41 per hour |
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Consultation
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Free |
Free |
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FACScan
/ Calibur/ No operator
FACScan / Calibur/ Operator provided |
$30
per hour
$55 per hour |
$38
per hour
$68 per hour |
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FACS ARIA Cell
Sorter / Operator provided
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$70
per hour |
$87 per hour
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Instrument
Setup Times
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| FACScan
Analyzer - Flat 0.25 hours |
FACS
ARIA |
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Non
sort single laser 0.5 hours |
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Non
sort multiple lasers 0.5 hours |
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Sort
nonsterile 1.0 hours |
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Sort
Sterile 1.0 hours
Analysis - multiple lasers 0.5 hours |
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Scheduling
Facility Time:
- Time on
the instruments maybe scheduled via phone or the internet once
the facility has been used by a particular investigator. See below.
ALL USE MUST BE SCHEDULED IN ADVANCE.
- Initial
use of the instruments can be scheduled only after a required
first time use consultative appointment has been made. This appointment
is free and is designed to ensure that FACS analysis/sorting will
meet the anticipated needs of the investigator and that there
will be minimal technical problems with regards to the FACS end
of the project.
- There is
no penalty for canceling an appointment. However, "no shows"
are charged a minimum of one hour service charge.
To schedule
via internet: Enter Investigator name, date of request, and estimated
number of samples to be analyzed. All efforts will be made to accommodate
your request. The more advance notice that is given helps ensure
that your requested time will be available.
Internet
Scheduling "not yet available-under construction"
To schedule
via phone - Call Flow Cytometry Laboratory at 444-8078
to schedule instrument time. Call Ms. Corrinne Leombruno at
444-8078 or Dr. Golightly at 444-2240
to schedule initial consultative appointment.
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