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CLINICAL FLOW LABORATORY

About the Laboratory... The Clinical Flow Cytometry laboratory at Stony Brook has been in existence since 1983. It is a full function laboratory which offers the latest in diagnostic Flow cytometry featuring;  Leukemia/Lymphoma phenotyping, residual disease determinations, stem cell quantitation, T cell subset analysis, DNA and ploidy analysis, Paroxysmal Nocturnal Hemoglobulinuria diagnosis and characterization, Cell Adhesion molecule analysis, Fetal hemoglobin determinations, and Granulocyte Function testing.

Clinical Laboratory Services

DNA/Ploidy Analysis
Fertility Testing - Sperm capacitation
Fetal Cells (Hemoglobin F) by Flow
Granulocyte Function (NBT tests) by Flow 
HLA B27 by Flow Cytometry
Leukocyte Adhesion Deficiency (LAD) evaluation
Leukocytes Analysis of Semen
Leukemia/lymphoma Phenotyping
Minimal Residue Disease testing
PNH diagnosis 
Reticulated Platelets
Stem Cell quantitation
T cell Subsets

Flow Cytometry Tutorials

Our Interactive Flow Cytometry Case studies,
phenotyping, analysis, and interpretation web page
Cytogenetic correlates in Immunophenotyping/ prognostics
Cytometric (multiplexed) bead arrays
Chronic Granulomatous Disease (CGD)
Fetal Cells (Hemoglobin F)
Minimal Residue Disease (MRD) detection
Paroxysmal Nocturnal Hemoglobinuria (PNH)
Plasma Cell Dyscrasias
T cell leukemia/lymphoma
Non Hematologic Malignancies
ZAP-70

 

 

Clinical Consultative Services - The Head of Immunology laboratories/Director of Flow Cytometry Core Facility is available to discuss Laboratory services regarding a particular patient in order to reduce costs and customize testing for the highest probability of obtaining clinically useful information.   Interpretative and technical consultative services are also available at no additional costs to the physician or patient. 
Dr. Marc Golightly Head of Immunology 631) 444-2240
Clinical Cytometry Laboratory (631) 444-8078

E-Mail Dr. Golightly

Visit Dr. Golightly's home page

last update 28-Feb-2007

Flow Cytometry Staff - ( Kerri, Nancy) (Todd) (Corinne, Nancy, Todd, Christopher, Kerri)

 
 

Fetal Cells (Hemoglobin F) by Flow Cytometry

Availability - Monday thru Friday 7:30am-11pm, for emergency call lab (631) 444-8078 or page Dr. Golightly
Specimen Requirement - At least 0.5 ml EDTA anticoagulated blood
Turn Around Time - Usually 24 hours for verbal, same day for emergencies (lab must be notified)
Test Information - This test replaces the Kleihauer-Betke test as the method of choice for detection of Fetal cells. It should be noted that the test also can detect and differentiate F-cells which may aid in the diagnosis of hereditary anemic diseases such as Sickle Cell, Hereditary Persistence of Fetal Hemoglobin, and Thalassemia. For more information and examples see tutorial on Fetal Cells (Fetal Hemoglobin F).


Granulocyte Function (NBT tests) by Flow

Availability - Monday thru Friday 7:30am-11pm, for emergency call lab 444-8078 or page Dr. Golightly
Specimen Requirement - At least 0.5 ml Herparin or EDTA anticoagulated blood
Turn Around Time - Usually 24 hours for verbal, same day for emergencies (lab must be notified)
Test Information -This test replaces the NBT test as the method of choice for diagnosis of Chronic Granulomatous Disease. It is very sensitive and can easily detect X-linked carriers. See for CGD tutorial more information and examples.


Leukemia/Lymphoma Phenotyping

Availability - Monday thru Friday 7:30am-11pm, for emergency call lab 444-8078 or page Dr. Golightly
Specimen Requirement - At least 1 ml Herparinized blood/bone marrow, FNA specimen, Tissue specimen
Turn Around Time - Usually 24 hours for verbal, same day for emergencies (lab must be notified)
Markers Performed - All leukemia/lymphoma studies are custom panels to ensure most accurate and cost effective diagnosis for the patient. Call Dr. Golightly (444-2240) to briefly discuss case and clinical impression. Results will be correlated with histology, hematology, and cytology when possible. See Interactive case studies for interpretation, examples, charts, etc.

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