Light Scatter	CD45/14

DR/CD34	CD11b/Glyco	CD13/15

CD33/TdT	MPO	CD3/CD7

Other markers performed but not shown which were negative; B cells (CD19, CD20, Kappa, Lambda), CD10(Calla).

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Explaination and interpretation of Flow cytometry results
Explaination of other results seen (histology, hematology,etc) and facts about this patient's disease

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Bone Marrow / Blood - characteristics/histology/hematology

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• The majority of cells in the bone marrow are of large in size by forward angle light scatter and with intermediate granularity (internal complexity) by 90 degree light scatter (see light scatter). This matches that seen by histology (see histology).

 
• The CD45/14 contour plot reveals that the majority of cells (86%) within the set light scatter gates are CD45 and CD14 positive. This indicates that the cell population in question expresses the monocyte/promonocyte antigen (LPS receptor). It should be noted that the expression of CD14 ranges from low intensity to high intensity which most likely indicates different maturational stages of CD14 expression. Combined with the light scatter and the high percentage of CD14 expression an M5-AML is suggested (see CD45/14).

 
• The DR/CD34 contour plot reveals that the majority of cells express DR but not CD34 (hemopoietic progenitor cell antigen). This is unusual for AML-M4/5 (most are positive). The Dr expression is expected on monocytes.  (see DR/CD34).

 
• The CD11b/Glycophorin contour demonstrates that there are few if any red cell precursors and that the majority of cells express CD11b (C3bi receptor) which is found on monocytes, granulocytes, and NK cells. (see CD11b/Glyco).

 
• The CD13/CD15 contour plot shows that the cells in question are somewhat heterogenous (again agreeing with histology) as the majority of cells express both CD13 & CD15, but there is a population which only express CD13. (see CD13/CD15).

 
• The CD33/TdT confirms the myeloid nature (CD33 positive) of these blasts and excludes a lymphoid (TdT negative) nature. (see CD33/TdT).

• The  Myeloperoxidase (MPO) (see MPO) negativity by flow cytometry confirms the MPO by special histology stains.

 
• All of these results when taken together suggest that this is an AML mostly likely an M5 or possibly an M4.  The percentage of CD14 is on the high end for what is expected for M4.  The distinction between M4 and M5 by flow cytometry is difficult and has not traditionally been done. See AML chart below.


Explaination of other results seen (histology, hematology,etc) and facts about this patient's disease