Light Scatter	CD45/14

DR/CD34	CD11b/Glyco	CD13/15

CD33/TdT	MPO	CD3/CD7

Other markers performed which were negative; B cells (CD19, CD20, Kappa, Lambda), and CD10(Calla)

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Explaination and interpretation of Flow cytometry results
Explaination of other results seen (histology, hematology,etc) and facts about this patient's disease

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Bone Marrow / Blood - characteristics/histology/hematology

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• There were two populations of cells in the bone marrow. The smaller cells were of lymphoid origin and were not remarkable. The large sized cells by forward angle light scatter have intermediate granularity (internal complexity) by 90 degree light scatter (see light scatter). This matches that seen by histology (see histology).

 
• The CD45/14 contour plot reveals that the majority of cells within the large cell light scatter gates are CD45 and CD14 positive (66%). However, there is a significant population (34%) which does not express CD14. These results indicates a heterogenous population with the majority expressing the monocyte/promonocyte antigen (LPS receptor). Combined with the light scatter this suggests an M4 AML (see CD45/14).

 
• The DR/CD34 contour plot reveals that the majority of cells express DR (67%) and some  express CD34 (31%)(hemopoietic progenitor cell antigen). This expression is expected on M4 AML.  (see DR/CD34).

 
• The CD11b/Glycophorin contour demonstrates that there are few if any red cell precursors and that the majority of cells express CD11b (C3bi receptor) which is found on monocytes, granulocytes, and NK cells. (see CD11b/Glyco).

 
• The CD13/CD15 contour plot shows that the cells in question are heterogenous (again agreeing with histology) as the cells express both CD13 & CD15, and there is a population which only express CD13. (see CD13/CD15).

 
• The CD33/TdT confirms the myeloid nature (CD33 positive) of these blasts and excludes a lymphoid (TdT negative) nature. (see CD33/TdT).

• The  Myeloperoxidase (MPO) (see MPO) positivity by flow cytometry confirms the MPO by special histology stains.

 
• All of these results when taken together suggest that this is an AML mostly likely an M4.  The percentage of CD14 is what is expected for M4.  The distinction between M4 and M5 by flow cytometry is difficult and has not traditionally been done. See AML chart below.

 

 
 
 

Explaination of other results seen (histology, hematology,etc) and facts about this patient's disease