Light Scatter	CD45/14

CD34/38	CD3/DR	CD10/TdT

CD5/20	CD19/Kappa	CD19/Lambda

Next to:
Explaination and interpretation of Flow cytometry results
Explaination of other results seen (histology, hematology,etc) and facts about this patient's disease

Back To:
CBC
WBC Differential
Bone marrow/Blood – characteristics/histology/hematology

Select:
A new case study
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

• The majority of cells in the bone marrow are of small to intermediate in size by forward angle light scatter and with very low granularity (internal complexity) by 90 degree light scatter (see light scatter). This matches that seen by histology (see histology).

 
• The CD45/14 contour plot reveals that the majority of cells within the set light scatter gates are CD45 negative. This indicates that the cell population is either immature (before CD45 is expressed), a plasma cell (CD45 negative), or of non-leukocyte origin. There are no monocytes/promonocytes present (see CD45/14).

 
• The CD34/38 contour plot reveals that the majority of cells express both the CD34 (hemopoietic progenitor cell antigen) and CD38 (on pre-B, plasma cells, thymocytes,& NK) which suggests a progenitor and when coupled with CD38 leaves only pre-B and thymocytes as possiblities (see CD34/38).

 
• The CD10/TdT contour plot confirms the early nature of this cell.  CD10 (Common acute lymphoblastic leukemia antigen - CALLA) as well as TdT (Terminal deoxynucleotidyl transferase) are positive in early progenitor cells (see CD10/TdT).

 
• The CD5/20 contour plot shows that the cell in question is not postive for the T cell antigen CD5 or for the mature B cell antigen CD20 (see CD5/20).

 
• The CD19/kappa and CD19/lambda contour plots demonstrates CD19 (B cell antigen expressed on pre-B cells to mature B cells) positivity while not expressing surface immunoglobulin of either light chain (see CD19/Kappa, CD19/lambda).

 
• All of these results when taken together suggest that this is a pre-B acute lymphocytic leukemia (ALL).  To demonstrate clonality only a few antibodies need to be run, however, to obtain information regarding the maturational stage of the cell, several antibodies with known maturational characteristics are needed.  This is demonstrated in the ontogeny chart below.

 

 
 
 
 
 

Explaination of other results seen (histology, hematology,etc) and facts about this patient's disease

Back To:
CBC
WBC Differential
Bone marrow – characteristics/histology

Select:
Case Index page to choose another case